Please upload a fasta file with protein sequences. Or download some example genomes: I. sakaiensis, P. aeruginosa, A. fumigatus . The file can be compressed as .gz. The example files consists of all proteins predicted fron the genome of these organisms.
If the uploaded file has protein sequences, use BLASTP. If it has nucleotide sequences, use BLASTX.
E-value (1e-x)
Expected number of chance matches in a random model. For example, a value of 6, means 1e-6. (Evalue tutorial video)
Suggested values: 3 - loose, 6 - medium, 20 - restrict.
Percent identity of the alignments.
Suggested values: 10 - loose, 30 - medium, 70 - restrict.